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From 2010.igem.org

isoprene production

In previous situations the hydrocarbons were degraded only to form biomass and CO2. It is interesting to see how much product could be made from hydrocarbons. Isoprene is a volatile product found in plants. E. coli will not be able to produce is in the near future, but it is an interesting product It is a very reduced product, with a similar amount of electron per carbon atom. Hydrocarbons have 6 - 6.3 electrons per carbon atom depending on the length and isoprene has 5.6 electron per carbon electron. If these values are close to each other, it has a positive influence on the maximal theoretical yield. Also the volatile nature of isoprene is very favorable for the downstream process

The pathway is displayed here http://biocyc.org/META/NEW-IMAGE?type=PATHWAY&object=PWY-6270&detail-level=2

In this scenario the lumped isoprene production pathway was added to metabolic network;

1 pyruvate + 1 D-glyceraldehyde-3-phosphate + 1 NADPH + 3 NADH + 3 ATP -> isoprene + CO2

isoprene export

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