Team:Queens-Canada/8 July 2010

PCR

Thai

pGpd-2, pFlp-1

Program run using HIFI with an extension time of 25 s and annealing temperature of 57 °C.

Heat Shock

Chris

pBluescriptII

Plates stored in 37 °C room overnight.

Gel Electrophoresis

Thai and Yuli

Used 0.4 g agarose in 60 mL TBE. Gel run for 45 mins at 100 V.

Gel Electrophoresis

Hao and Chris

Used 0.4 g agarose in 60 mL TBE. Gel run for 45 mins at 100 V.

Digestion

Yuli

BBa_I13522

Cut using EcoRI and PstI.

Microinjection

Hao and Yuli

Practiced injections with water.