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Team:Queens-Canada/30 June 2010
From 2010.igem.org
June 30, 2010
PCR Stitch
Hao and Steve
pIC576, pIC518
Glycerol Stock
Thai
BBa_I13522
Socked liquid culture from 29/06/10 into -80 °C freezer.
Miniprep
Thai and Hao
BBa_I13522
Standard procedure followed.
Gel Electrophoresis
Hao and Steve
pIC576, pIC518
Used 0.35 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V.
PCR
Thai
eCFP (pIC517)
Program run using TAQ with an extension time of 45 s and annealing temperature of 56 °C.
Gel Extraction
Yuli and Steve
mCherry
Extracted lane 4 from Gel on 30/06/10.
Gel Electrophoresis
Yuli
eCFP/pIC517, pIC518-1a
Used 0.2 g agarose in 40 mL TBE. Gel run for 35 mins at 100 V.
Gel Extraction
Louis and Yuli
eYFP-1a
PCR
Louis and Yuli
eYFP-1a and eYFP-2a
Program run using HIFI with an extension time of 60 s and annealing temperature of 61 °C.
