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Team:Queens-Canada/28 June 2010
From 2010.igem.org
June 28, 2010
PCR
Steve
pSip-1, pMec-7, pRab-7
For pSip-1 and pRab-7: Program run using HIFI with an extension time of 12 s and annealing temperature of 58 °C.
For pMec-7: Program run using HIFI with an extension time of 30 s and annealing temperature of 56 °C.
Gel Electrophoresis
Basia and Yuli
pSip-1:::eGFP Extract, pRab-7:::eGFP Extract, pMec-7:::eGFP Extract, unc-54 3'UTR
Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.
Microinjection
Hao and Yuli
Practiced injections with water.
