Team:Queens-Canada/22 July 2010

Gel Electrophoresis

Steve

eYFP Extract

Used 0.7 g agarose in 100 mL TBE. Gel run for 50 mins at 100 V.

Miniprep

Hao and Thai

PRF4

Sequencing Prep

Chris and Thai

pSip-1::1A3

Used 4 µL of 2µM primers with 8 µL of plasmid.

PCR

Yuli

pSip-1:::eGFP, pRab-7:::eGFP, pMec-7:::eGFP, unc-54 3'UTR, pStr-1

Program run using 9:1 FAST:HIFI blend with an extension time of 45 s and annealing temperature of 61 °C.

Gel Electrophoresis

Chris

pSip-1:::eGFP, pRab-7:::eGFP, pMec-7:::eGFP, unc-54 3'UTR, pStr-1

Used 0.3 g agarose in 50 mL TBE. Gel run for 45 mins at 100 V.

PCR

Steve

pSB1A3

Program run using 9:1 FAST:HIFI blend with an extension time of 70 s and annealing temperature of 62 °C.

PCR

Yuli

eYFP1a and eYFP2a

Program run using 9:1 FAST:HIFI blend with an extension time of 45 s and annealing temperature of 58 °C.