Team:HokkaidoU Japan/Notebook/September22

From 2010.igem.org

Notebook

Digestion of pSB1A3, Arabinose Promoter & GFP + double terminator

  • Digestion mix -> XbaI in GFP + double terminator solution required putting on incubator for a long time, so did it.
Reagent Amount
10x H buffer 2 uL
DW 10
pSB1A3 15
0.1% BSA 2
EcoR I 0.5
Pst I 0.5
Total 30 uL
Reagent Amount
10x H buffer 2 uL
DW 8
Arabinose Promoter 6.3
0.1% BSA 2
EcoR I 1
Spe I 0.7
Total 20 uL
Reagent Amount
10x M buffer 5 uL
DW 32.5
GFP + double terminator 3
BSA 5
Xba I 4
Pst I 0.5
Total 50 uL
  • Precipitated two samples by EtOH after extracted them from a gel.
->Ligation & Transformation
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