Team:EPF Lausanne/Calendar/0727

From 2010.igem.org


27 July 2010

  • Ligation C3/A3 + Ori + resistance (Kan, Amp, Tet, Cm)
  • transformation of the ligation products into E.Coli DH5a
  • Plating of transformed cells with selection of the vector and the insert
  • Colony PCR of E.Coli DB3.1 containing BaseVector + Ori + Kan --> running gel (twice, the first time failed)
  • Preculture of (maybe!!) WT Asaia for competence protocol
  • Sorting of male/female flies
  • Culture of 400 ml of different species of bacteria.
Previous day Next day