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From 2010.igem.org

Tony and Matt Ran gel of AOX and CAT genes. Reaction successful. Also ran colony PCR in order to confirm appearance of pucBB-DAS colonies. Ian PCR reaction of psB3K3 pLac-GFP. Sent psB3K3 Eut SK for sequencing to University of Minnesota BioMedical Genomics Center. Matt Nanodrop results of AOX and CAT PCR. Ligase free cloning of AOX/DAS PCR products with pucBB NotI/BglII. Transformed and placed plates in thirty seven degree overnight.