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From 2010.igem.org

Annie Inoculated four microliters Pichia in fifty microliters YPD media. Plated on YPD media for future inoculation. Tony and Matt Colony PCR screen to find pacBB EutG DsRed clones from strawberry background. Four hundred fifty microliter reactions. Ian Minipreps of psB3K3 Eut LKMNS. Gel purification of pLac/SpeI PCR product. Digestion reaction of psB3K3 Eut LKMNS reaction.