Toronto/4 August 2010
From 2010.igem.org
-Received fusion primers in mail
Resuspended the enzyme DNA:
-Spinned the 6 tubes briefly ( a few seconds ~10s) to ensure all the contents were on the bottom of the tube
-Added 50uL of sterile water to each tube
-Vortexed for ~2sec each
-Kept on ice for 5-10min
-vortexed lightly again-->stored in the -20C fridge in the white DNA box.