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From 2010.igem.org

Jocelyn:

Pick and amplify the single colony from the promoter-pdu LB plate. 1 each (prep for protein expression)

Miniprep of the promoter-pdu. 5 in all

PduAB construction failed. Forgot to add the mid-part. T.T => Double Digest plasmid again.

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Hilde:

Fastdigest text: plm. of GFP & RFP

Colony PCR test (VR2,ABr3)

Fastdigest cutting: Promotor(Insert,E,S) & ABJKNTUV(Vector,E,X)