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From 2010.igem.org

Knightmare：

Plasmid extraction: ABJKNTUV on PSB1C3 in old standard with concentration and digestion determination.

Jocelyn:

Double Digest ABJKNTUV on PSB1C3 and Promoter. (0.5h & 1h)=>Ligation =>transformation into TOP10

Construct pduAB under new standard.(condition tests:3h+PCR recycle+1h)=>ligation=>transformation into TOP 10(on 9.12)

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Hilde:

Ligation of pduABJK-pduNTUV

Fastdigest cutting of pduNTUV (X,P) & ABJK(S,P)

Ligation of pduABJK-pduNTUV