Team:TU Delft/1 July 2010 content

From 2010.igem.org

Lab Work

Pseudomonas Putida GPO1

The second day of the grow process of Pseudomonas Putida GPO1

Sample Medium day 1 = 30 June day 2 = 1 July
no cells E2 without carbon 0.00 0.00
no cells LB 0.00 0.00
no cells E2 + 1% LB 0.00 0.01
Pseudomonas Putida GPO1 * E2 + 1% LB 0.01 0.05
no cells E2 + 10% LB 0.00 0.00
Pseudomonas Putida GPO1 * E2 + 10% LB 0.34 0.19
no cells E2 + 1% octane 0.00 0.00
Pseudomonas Putida GPO1 * E2 + 1% octane 0.00 0.00
Pseudomonas Putida GPO1 * E2 + 10% octane 0.29 0.18

* average of 3 colonies

Pseudomonas Putida GPO1 can grow on 10% octane as carbon source.

Competent cells

Top10 cells were cultivated for making competent cells.