Team:Stockholm/25 October 2010
From 2010.igem.org
Mimmi
SOD activity
- continuing
mix | samples | blank 1 | blank 3 |
---|---|---|---|
sample solution | 20 | ||
ddH2O | 20 | 20 | |
WST solution | 200 | 200 | 200 |
Enzyme solution | 20 | 20 | |
dilution buffer | 20 | ||
tot | 240µl | 240µl | 240µl |
- Incubate in 37°C for 20 min
- Measure A440 with nanodrop
- SOD activity (inhibition %) = ((Ablank1 - Ablank3) - (Asample - Ablank2))/(Ablank1 - Ablank3) x 100
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