"

From 2010.igem.org

Mimmi

SOD activity

- continuing

mix	samples	blank 1	blank 3
sample solution	20
ddH2O		20	20
WST solution	200	200	200
Enzyme solution	20	20
dilution buffer			20
tot	240µl	240µl	240µl

• Incubate in 37°C for 20 min

• Measure A₄₄₀ with nanodrop

• SOD activity (inhibition %) = ((A_blank1 - A_blank3) - (A_sample - A_blank2))/(A_blank1 - A_blank3) x 100