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Team:Queens-Canada/31 August 2010
From 2010.igem.org
August 31, 2010
Miniprep
Hao and Thai
pOsm-10::eCFP::unc-54 3'UTR::1A3, pSra-10::eCFP::unc-54 3'UTR::1A3
Digestion
Hao and Thai
pOsm-10::eCFP::unc-54 3'UTR::1A3, pSra-10::eCFP::unc-54 3'UTR::1A3
Gel Electrophoresis
Hao and Thai
pOsm-10::eCFP::unc-54 3'UTR::1A3, pSra-10::eCFP::unc-54 3'UTR::1A3
Used 0.7 g agarose in 100 mL TBE. Gel run for 30 mins at 100 V.
Digestion
Hao and Chris PRF4, eYFP, eGFP
Gel Electrophoresis
Hao and Chris PRF4
Used 0.3 g agarose in 40 mL TBE. Gel run for 40 mins at 100 V.
Ligation
Hao and Chris
eGFP::unc-54 3'UTR::1A3, eYFP::unc-54 3'UTR::1A3, ChR2::unc-54 3'UTR::1A3
| EcoRI and SpeI | XbaI and PstI | EcoRI and PstI |
| eYFP | unc-54 3'UTR | 1A3 |
| eGFP | unc-54 3'UTR | 1A3 |
| mCherry | unc-54 3'UTR | 1A3 |
Backbone treated with CIAP for 30 mins.
Heat Shock
Hao and Chris
eGFP::unc-54 3'UTR::1A3, eYFP::unc-54 3'UTR::1A3, mCherry::unc-54 3'UTR::1A3
