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Team:Queens-Canada/17 August 2010
From 2010.igem.org
August 17, 2010
Gel Electrophoresis
Thai and Hao
Used 0.7 g agarose in 100 mL TBE. Gel run for 45 mins at 100 V.
PCR
Yuli
pSip-1::eGFP, pRab-7::eGFP, NpHR::eCFP
Program run using 9:1 FAST:HIFI blend with an extension time of 5 s and annealing temperature of 60 °C.
Gel Electrophoresis
Yuli and Thai
pSip-1::eGFP, pRab-7::eGFP, NpHR::eCFP
Liquid Culture
Chris
pRab-7, mCherry, pFlp-1 (all in 1C3)
Topped up Liquid Culture tubes with 1.5-2 mL 2XTY + Chlor.
Heat Shock
Chris and Mary
eYFP, pStr-220, pMec-7, pSra-10, pHsp-3, pSrb-6 (all in 1C3)
Microinjection
Hao
Practiced injections with Sur-5::GFP marker.
