Experiments

Lab notebooks (click the following links to see details):

Experimental results:

Speedy switch results: Several reporter gene assay results for testing the Theophylline riboswitch.
Speedy degrader results: Several reporter gene assay results for testing the half life of various ssrA tagged and untagged fluorescent proteins.

Protocols, preparation and other stuff

Basic cloning protocols. Optimised protocols developed and kept current over the past few years.
Gene reporter assay protocols

The range given to each group for this years' parts was divided as follows:

The designed parts: <groupparts>iGEM010 NYMU-Taipei</groupparts>

This is a list of all the primers we designed for

name	sequence	length
eIF4A_split_A_Fp	cggcagcagcggcagcggcagcATGGAGCCGGAAGGCGTCATCGA	45
eIF4A_split_B_Rp	ctgcagcggccgctactagtaTCAAATGAGGTCAGCAACGTTGAG	45
GFP_split_A_FP	gaattcgcggccgcttctagagatgcgtaaaggagaagaacttttc	46
GFP_split_B_RP	ctgccgctgctgccgctgctgccttattatttgtatagttcatccatgcca	51
GFP_split_A_RP	gctgccgctgctgccgctgctgccttgtttgtctgccatgatgtatac	48
GFP_split_B_FP	gctgccgctgctgccgctgctgcctttgtatagttcatccatgccatg	48
aptamer_FP	gcttctagagacactcggaggacagcttagatgcaaagccggagtgagtgtacacc	56
aptamer_RP	agcctgcagcggccgctactagtattcccctggcgcggggtgtacactcactccggct	58
eIFA_FP	gcttctagATGGAGCCGGAAGGCGTCATCGA	31
eIF4A_mut1_FP	ccatatcaattctccagcagattga	25
eIF4A_mut1_RP	caatctgctggagaattgatatggc	25
eIF4A_mut2_FP	gcagaagctccagatggaa	19
eIF4A_mut2_RP	tccatctggagcttctgca	19
eIF4A_split_A_Rp	ctgcagcggccgctactagtatcaagggtctctcataaatttcttgg	47
eIF4A_split_B_Fp	cggcagcagcggcagcggcagcattcggattcttgtcaagaagg	44
RFP_split_A_FP	gaattcgcggccgcttctagatggcttcctccgaagac	38
RFP_split_A_RP	gctgccgctgctgccgctgctgccgtcttccgggtacatacgtt	44
RFP_split_B_FP	gaattcgcggccgcttctagatgggtgctctgaaaggtgaaatc	44
RFP_split_B_RP	gctgccgctgctgccgctgctgccagcaccggtggagtga	40
Total		773

name	sequence	length
Theophylline Riboswitch FP	gaattcgcggccgcttctagagggtgataccagcatcgtcttgatgcccttggcag	56
Theophylline Riboswitch RP	ctgcagcggccgctactagtacttgttgtcttgcagcggggtgctgccaagggcatcaagac	62

name	sequence	length
GFPLVA_out_FP	gcttctagagaaagaggagaaatactagatgcgtaaaggagaagaacttttc	52
RFPLVA_out_FP	gcttctagagaaagaggagaaatactagatggct	34
RFPLVA_in_RP	CTACTAAAGCGTAGTTTTCGTCGTTTGCAGCagcaccggtggagtga	47
SspB_FP	gcttctagatgGATTTGTCACAGCTAACAC	30
SspB_RP	ctgcagcggccgctactagtattaCTTCACAACGCGTAATGC	41
RFP_FP	gcttctagatggcttcctccgaagac	26
GFP_FP	gcttctagatgcgtaaaggagaagaacttttc	32
xFP_LVA_Remover_FP	gagctgtacaagaggccttaataatactagagccaggcatca	42
xFP_LVA_Remover_RP	tgatgcctggctctagtattattaaggcctcttgtacagctc	42
Total		346