Team:HokkaidoU Japan/Notebook/August31

From 2010.igem.org

Notebook

Gel extraction of pUC119, Ligation and Transformation

  • Added 10 uL 6x Sample Buffer to pUC119 solution 50 uL
  • Divided among 3 lanes
  • Gel extraction (gel weight: 220 mg)
  • Final volume after extraction was50 uL（500 ng/uL）

Ligation

Empty I M
DNA 2 uL 2 uL 2 uL
ligation solution I － 2 uL －
Mighty Mix － － 2 uL
T4 ligase － 0.4 uL 0.4 uL
Total 2 uL 4.4 uL 4.4 uL
→Transformation
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