Team:Cambridge/biobrickingoligosfirefly
From 2010.igem.org
1 ttcgctaaggatgatttctg gaattcgcggccgcttctag ag Tm:70.14 dG:1.54
2
ttgcccgtttttttgccgga ctgcagcggccgctactagta
Tm:68.73
dG:-0.26
3
tactagtagcggccgctgcag
Tm:68.73
dG:0.57
4
ctctagaagcggccgcgaatt
Tm:70.55
dG:0.67
5
gaattcgcggccgcttctagagCGCCGGTGTGAAAGTTAAGCT (borrows some of the PP.LRE to get a close melting temp)
Tm:67.53
dG:-0.39
6 ctgcagcggccgctactagtaTTACAGCTTAACTTTCACACCGGC Tm:67.18 dG:0.41
7 GCCGGTGTGAAAGTTAAGCTGTAAtactagtagcggccgctgcag Tm:67.18 dG:0.57
8 AGCTTAACTTTCACACCGGCGctctagaagcggccgcgaattc Tm:67.53 dG:-0.54
9 gaattcgcggccgcttctagagGGGTGACCGTGTTAAGCTGT Tm: 64.34 dG:0.46
10 ctgcagcggccgctactagtaTTACAGCTTAACACGGTCACCC Tm:65.21 dG:0.41
11 GGGTGACCGTGTTAAGCTGTAAtactagtagcggccgctgcag Tm:65.21 dG:0.57
12 ACAGCTTAACACGGTCACCCctctagaagcggccgcgaattc Tm: 64.34 dG: 0.67