"

From 2010.igem.org

• According to [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WB5-4DXBGFJ-D5&_user=1495569&_coverDate=05%2F31%2F1960&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000053194&_version=1&_urlVersion=0&_userid=1495569&md5=8d7118ece7966e711eb16425e94285cf "Spectral emission and quantum yield of firefly bioluminescence"(1960)] (H. H. Seliger and W. D. McElroy)

• 1. The emission spectrum of in vitro firefly bioluminescence has been found to be identical with the in vivo bioluminescence.
• 2. One light quantum is emitted for every luciferin molecule oxidized, the yellowgreen emission band peaking at 562 mμ.
• 3. A low quantum yield red emission appears in acid pH and at high phosphate buffer concentrations even at neutral pH.
• 4. An energy stabilization role is postulated for the luciferase molecule in view of the high quantum yields measured.

• 562 um peak in radiation

• [http://www.nature.com/nphoton/journal/v2/n1/abs/nphoton.2007.251.html Firefly bioluminescence quantum yield and colour change by pH-sensitive green emission(2008)]

• [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4DYVHVW-98&_user=1495569&_coverDate=07%2F31%2F1959&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000053194&_version=1&_urlVersion=0&_userid=1495569&md5=35a7850f2cd36792ddfd94466f88c43d Quantum yield in the oxidation of firefly luciferin (1959)] .88plusminus.25