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From 2010.igem.org

Annie Minipreps of pac and GFP. Overnight digest of DsREd Eut C and Eut G with BglII/NotI. Matt Digestion of pacBB-GFP with BglII/NotI (fifty microliter reaction). Placed in thirty seven degree incubator overnight. PCR reaction of eutG-GFP (three hundred microliters.