"

From 2010.igem.org

Annie Made YDP media and inoculated PIchia Pastoris. Tony PacBB GGFP digest with BglII/NotI. Cut out top band and saved for purification tomorrow. Transformation of pacBB Eutc DsRed ligation into JM109. Matt Minipreps of pacBB EutC DsRed. Tony Preparation of pacBB EutC DsRed for sequencing. Overnight cultures of psB3K3 Eut LKMNS.