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Korea U Seoul/15 September 2010
From 2010.igem.org
PCR : promoters and reporter genes
ERROR FOUND!! One more round!
- PCR : PyodA-mAAA, PzntA-RFP(BBa_E1010) and ParsR-GFP(BBa_E0040)
| Reagent | Volume (μL) |
| 2.5mM dNTP | 3 |
| 10x buffer | 5 |
| Plasmid template (20ng/μL) | 2 |
| Primers (10pmole/μL) | 4 |
| α-Taq DNA polymerase (5U/μL) | 0.5 |
| D.W. | 35.5/total=50 |
95˚C(2’)-[95˚C(20”)-55˚C(20”)-72˚C(2’)]30-72˚C(5’)-4˚C | |
