Our screening software must be able to uncover hidden SAT sequences within benign ones. To verify if our software is really efficient to find them, we have created intervening sequences. These are non select agent sequence modified with the addition of a select agent sequence within the sequence. For that we selected select agent sequences of different length: 150bp, 200bp, 250bp, and flanked them on both sides by contiguous 300bp sequences originating from GenBank entry NM_001006133.1(Gallus gallus protein kinase C, delta (PRKCD), mRNA). We obtained like that sequences of length 750bp, 800bp, 850bp, 50 for each length. The select agent sequence introduced were from select agent sequence flagged by our software.

According to the guideline, only sequences over 200bp should be flagged, so the results expected on this test are a hit for 200bp and 250bp intervening sequences, and not a hit for 150bp intervening sequences.

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