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From 2010.igem.org

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• 16:14, 3 August 2010 Jdchoo (Talk | contribs) uploaded "File:07.30.2010.JeremypRFPGradientSuccess copy.jpg" ‎ (Jeremy's Gradient PCR of biobricking pRFP. This used forward primer EcoRI, XbaI and gene specific region and reverse primer was gene specific and SpeI. )