Team:ETHZ Basel/Biology/Implementation
From 2010.igem.org
Experimental results
generation of fusion proteins
The image shows all the fusion proteins that were planned. The BioBricks for all these constructs were generated via PCR or synthesized at GeneArt and ligated into the storage vector for sequencing. Easy cutting and pasting into working vectors should be possible with the cloning strategy BBF RFC 28.
Modeller's input
Due to the high amount of fusion proteins that were in planing, priorities had to be distributed to the different genes. This was possible due to the various models the dry-lab team implemented.