Team:Harvard/allergy/parts
From 2010.igem.org
parts and primers
Allergy
For more information on the allergens visit our "meet the allergens" pageName | Original Name | Description | Resistance | Sequence |
A1 | amiRNA GFP | GFP knockdown construct | chloramphenicol | amirnaGFP |
A2 | amiRNA (Other allergen x) | knockdown construct for x | chloramphenicol | amiRNA x |
A3 | ihpRNA Bet | Intron hairpin Bet Knockdown construct | chloramphenicol | ihpRNA Bet1 |
A4 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | chloramphenicol | ihpRNA LTP |
A5 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | chloramphenicol | ihpRNA GER |
Primers
Name | Description | Sequence |
AP1 | 1 of 4 primers used to create the amiRNA GFP construct | primer1 |
AP2 | 2 of 4 primers used to create the amiRNA GFP construct | primer2 |
AP3 | 3 of 4 primers used to create the amiRNA GFP construct | primer3 |
AP4 | 4 of 4 primers used to create the amiRNA GFP construct | primer4 |
AP5 | 1 of 4 primers used to create the amiRNA Bet construct | |
AP6 | 2 of 4 primers used to create the amiRNA Bet construct | |
AP7 | 3 of 4 primers used to create the amiRNA Bet construct | |
AP8 | 4 of 4 primers used to create the amiRNA Bet construct | |
AP9 | Forward primer for Bet v1 Sense | |
AP10 | Reverse primer for Bet v1 Sense | |
AP11 | Forward primer for Bet v1 Antisense | |
AP12 | Reverse primer for Bet v1 Antisense | |
AP13 | Forward primer for LTP Sense | |
AP14 | Reverse primer for LTP Sense | |
AP15 | Forward primer for LTP Antisense | |
AP16 | Reverse primer for LTP Antisense | |
AP17 | Forward primer for Ger Sense | |
AP18 | Reverse primer for Ger Sense | |
AP19 | Forward primer for Ger Antisense | |
AP20 | Reverse primer for Ger Antisense | |
AP21 | Forward Primer for PDK intron | |
AP22 | Reverse Primer for PDK intron | |
AP23 | Forward Primer to make amiRNA Biobrick compatible | |
AP24 | Reverse Primer to make amiRNA Biobrick compatible | |
AP25 | 1 of 4 primers used to create the amiRNA LTP construct | |
AP26 | 2 of 4 primers used to create the amiRNA LTP construct | |
AP27 | 3 of 4 primers used to create the amiRNA LTP construct | |
AP28 | 4 of 4 primers used to create the amiRNA LTP construct |