Team:Harvard/allergy/parts
From 2010.igem.org
parts and primers
Allergy
For more information on the allergens visit our "meet the allergens" pageName | Original Name | Description | Resistance | Sequence |
A1 | amiRNA GFP | GFP knockdown construct | chloramphenicol | amirnaGFP |
A2 | amiRNA (Other allergen x) | knockdown construct for x | chloramphenicol | amiRNA x |
A3 | ihpRNA Bet | Intron hairpin Bet Knockdown construct | chloramphenicol | ihpRNA Bet1 |
A4 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | chloramphenicol | ihpRNA LTP |
A5 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | chloramphenicol | ihpRNA GER |
Primers
Name | Description | Sequence |
AP1 | 1 of 4 primers used to create the amiRNA GFP construct | primer1 |
AP2 | 2 of 4 primers used to create the amiRNA GFP construct | primer2 |
AP3 | 3 of 4 primers used to create the amiRNA GFP construct | primer3 |
AP4 | 4 of 4 primers used to create the amiRNA GFP construct | primer4 |
VP7 | HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert | |
VP8 | smGFP-NheI reverse primer for Reporter Series 1 insert | |
VP9 | Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert | |
VP11 | Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert | |
VP12 | 1 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
VP13 | 2 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
VP14 | 3 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
VP15 | 4 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
VP16 | 5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
VP17 | 1 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
VP18 | 2 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
VP19 | 3 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
VP20 | 4 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
VP21 | 5 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
VP22 | 6 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
VP23 | 7 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
VP24 | 8 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
VP25 | 9 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
VP26 | 1 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
VP27 | 2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
VP28 | 3 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
VP29 | 4 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
VP30 | 5 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
VP33 | 1 of 2 for sequencing V7 and V8 biobrick cloning site | |
VP34 | 2 of 2 for sequencing V7 and V8 biobrick cloning site |