Team:DTU-Denmark/BBrick Characterisation

Mainly the hard control of the switch is due to a double regulation system build on a both terminator-anti-terminator and repressor anti-repressor regulation. It was out of the scope of this project to construct the entire theoretical developed switch, and characterize the fully constructed switch. Have focused on characterizing the two regulatory systems individually. This was done in order to investigate if the responses were satisfactory to use in a future complete switch construction. (???? By getting the regulatory mechanism of the subparts we further, by modeling, could conclude constraints for successful function of the system and other subparts. In this section we describe the design of and the experimental setup used to characterize the subparts of the system and our bio-bricks.

Anti-Terminator

Introduction to this part

Selecting subparts

Why were these subparts chosen ?
AIM

Design and experimental setup

presentation - Figure of setup and explanation

Materials and methods

HOW ? what plasmids and why, what measurering method and why?
refer to the notebook page with protocols - and actual info from lab.
mRNA-stability
when introducing non-coding sequences problems will acour with rna-degredation of RNAP is not attracted to the area, to fast degredation, unwanted steam loops. (Reference to the terminator screening plasmids for BB)

Results

comments to the results and reference to the BB pages with info and results.

Repressor function