Main Experimental Results

Characterising the pRS414 related components of the switch
Timed Induction of the Cup1 promoter using N4
Copper Dose Response of the Cup1 promoter using N4

Characterising the pRS415 related components of the switch
Galactose Dose Response of Gal1 promoter in pRS415
Timed Induction of Gal1 promoter in pRS415

Characterising the mutual inhibition whithin the switch
MS2 coat-protein effect on expression of GFP in pRS415

Troubleshooting pRS414
Experimental Layout

Characterising Biobrick E2050 mOrange Fluorescent Protein
Homologous Recombination of E2050 into pRS415 Construct in Place of GFP Protein
FACS Analysis of mOrange recombinant pRS415