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Team:Groningen/9 September 2010
From 2010.igem.org
Assembly of biobricks in pSB1C3-backbone for submission to parts registry by Maarten
O/N cultures of transformants were mini prepped with Sigma Aldrich mini prep kit and control restrictions were performed.
Control restrictions with EcoRI and PstI on constructs:
| 2 µL construct |
| 2 µL Fast Digest Buffer |
| 0,5 µL EcoRI Fast Digest |
| 0,5 µL PstI Fast Digest |
| 15 µL MQ |
| Construct | Expected band sizes (bp) |
|---|---|
| pSB1C3-C | 2031, 848 |
| pSB1C3-DC | 2031, 872 |
| pSB1C3-E | 2031, 317 |
| pSB1C3-H | 2031, 302 |
| pSB1C3-Sortase | 2031, 689 |
| pSB1C3-RFP | 2031, 1069 |
| 1-3 | 4-6 | 7-9 |
| Chp C | Chp DC | Chp E |
| 1 & 2 | 4-6 | 8 |
| Chp H | Sortase | RFP |
The gels were not very conclusive but samples in lane 3, 5 and 7 in the first gel and lane 1 and 6 in the second gel were sent for sequencing on 15 September.
Peter
Chaplin detection
Samples from the dry freezer were prepared using TFA.
The samples were diluted with 1 mL Q-H20 and put on gels:
Gel 1: chppt=e deltapt=e bpte e1pte enpte chste deltaste bste e1ste
Gel 2: enste deltapt=4 bpt=4 e1pt=4 enpt=4 deltast=4 bst=4 ent=4
Gel 3: est=4 deltadt=e bdt=e e1dt=e endt=e 1 2 3
Gel 4: 4 e1dt=e bdt=e e1nt=e e1pt=e bpt=e e1st=e b1st=e
