Team:Groningen/1 June 2010

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iGEM Groningen 2010

Hydrophobofilm
pushing coatings into a greener future

We've started working on the wiki, been preparing plasmids and are settling in our office space. Note to self: should have brought coffee machine


Week 22

The biobrick prefix and suffix are introduced into the pNZ8901 expression vector using PCR and primers with 5’ overhangs.

For optimization taq polymerase (fermentas) is used first 

Component	µl	Final concentration
Primer pNZ89bbs-for1	5	300nM
Primer pNZ89bbs-rev1	5	300nM
10x Taq buffer(-MgCl2)	5	1x
dNTP’s	2	200µM
MgCl2	3	1.5mM
Template	0.5	~14ng
Taq	0.5	2.5u
MQ	29	</pre

- 94°C,	 3’
	- 94°C,  30’’
30X	- 50°C,  30’’
	- 72°C,  1’30’’
- 72°C,  10’

[[Image:03-06-10 gn.jpg|200px|thumb|left|alt text]] 
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Week 23




















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