"

From 2010.igem.org

Home

The Team

The Project

Parts submitted

Modelling

Notebook

Blog

Safety Protocols Lab Protocols

Captain's log 040610 Maya, Thomas, Patrick, Lisa and Annemi have been working on a project description for Sebastien. The document 'project description' can be found in dropbox in the research group folder. From now on this document will be the only one describing the project, therefore you should edit in the document and not create a new one. We have decided that every time we are doing something iGEM related, that it should be posted in this document. Please write the date and your name at the beginning of your log. This will make it easier for all of us to keep track of what is going on in the group. We have tried to make a simple plan of what needs to be done before going to the lab: Investigate the E. coli chromosome to find out where we can integrate the switch Find the BioBricks we will be using (DNA sequences) Install the software CLC main workbench (the program which will be useful for the design of the system) Design the system: Order of genes, restriction sites, DNA sequences, order of integration of genes into the plasmids Which plasmids (Sebastien) Primer design How to test that the genes have been inserted (antibiotic resistance) Make a phase planning and talk to Peter (divide into smaller parts and and test these individually) Maya will start creating an illustration for the system during the weekend. Thomas will delete all irrelevant budgets from the dropbox. He will also send the project description to Sebastien.

Lab Work and Notebook

On this page is described the experiments, procedures and protocols that we have used.
Further the Results from relevant experiments are presented. We have written succeses as well as failures to share our experience, knowledge and tips n' tricks we learned while working with our BBrick parts and the BB-standards and methods.

Protocols
For all the methods we have shaped the protocols to the standards and experiences of our lab. We have collected the protocols we used in a comprehensive list below where it is possible to read them in full length. They contain our procedure as well as references. Some time this might be weakly documented when given to us by communication with supervisors.

Generel experiences

General experiments and procedures that both groups have used.

Biobrick assemply standards

standard.
3A.

PCR

Repressor group

more specific characterizing experiments the biobrick

Work flow

XXXX maybe show pictures of our work plan, or write it simplified - the more pictures the better XXX

Experiment I

what method what results

Experiment II

what method what results

Terminator group

XXXX here we should write a short abstract XXXX

Work flow

XXXX maybe show pictures of our work plan, or write it simplified - the more pictures the better XXX

Experiment I

what method what results

Experiment II

what method what results

Protocols

List of the protocols we used, and links to the word documents We should have references in our protocols, when available.

References and resources

• xxxxxxxxxxxx