What is a switch?
what is a biological switch, examples and existing constructions, What can we use it for. what have been build. Many small cuircuits have been constructed. And reviews have been done on also trying to build regulatory function in enzymes. See article (“Designing switchable enzymes” Marc Ostermeier) 3>What is a switch?Design and engineering of bi[o]stable
The THEORETICAL overall aim and vision
(THE bigger picture and STORY)
APPLICATIONS
how we designed our switch - selection of parts and parameters - and last presentation of our system.
Applications
Uncertainties and potential problems:
By designing the switch we did not know the exact distance needed from the nut-site to the terminator steam loop for proper function of anti-termination. We have taken the sequence form the natural seting and made it small enough to give sense as a biological building block.
We have tried to utilize the phage regulation to construct a biological switch that can be used in biological engineering.
When considering how to characterize the subparts of our system we looked at the work already done to characterize terminator efficiency. The screening plasmids made by (REFF) endy and XXX and XXX. The work clearly demonstrates the problem by creating a weldefined data sheet system, the data achived in terms of terminator efficiency is not consistent, and shows the complexity of biology.
It has not been possible for us do all the test needed to develop a wel defined switch system. Below is outlined our approach and in the end we suggest other approaches and possibilities for further work, and considerations in relation to this.
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