BIOTEC Dresden/Notepad/1 October 2010

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Assembly of the amplifier-reporter part

Part 45 (gel purified after restriction) was ligated into the linearized vector containing part 46 using the standard assembly method. The final aim is to ligate the product with part one in the order 1-45-26 to produce the amplifier-reporter:

ptetR RBS LuxR Stop1 Stop2 luxRp RBS RFP RBS-luxI-Stop1-Stop2

Ligation product showed the expected length in a restriction analysis also sequencing results expected.

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