Team:HokkaidoU Japan/Notebook/October4

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Notebook

Sequencing of 2 samples, RBS + Arabinose

9:00
  • Reserve the sequencer for use at 16:00 if posible
12:10
  • Consult colonie PCR to choose constructs for Sequencing.
  • Miniprep
  • MasterMix
  • PCR for sequencing, use 100 up and 200 down primers
12:55~14:55
  • PCR
15:00~15:40
  • Ethanol precipitation
  • Melting in Hi-DI
16:00
  • Start sequencing
17:00
  • Retrieve sequence
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