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OCTOBER: WEEK 1
October, 3rd
Glycerol stock for:
Pick of a single non-red colony of INTEIN_C3 and inoculum into 5 ml LB+Cm34. Sample was let grow ON at +37°C, 220rpm.
Miniprep and quantification of:
- I55-1: 57,4 ng/ul
- I55-1bis: 79,6 ng/ul
I55-1bis was digested E-S for about three hours and than gel run/cut.
Colony PCR for I74 (six colonies were picked)
Gel run for PCR samples and I55 (E-S) and cut for the last one.
 I74 screening and I55 E-S cut.
Gel extraction and quantification:
Inoculum of I35 into 5 ml LB+Amp: last quantification after gel extraction was too poor. We eill digest it again E-P.
October, 4th
October, 5th
October, 6th
October, 7th
October, 8th
October, 9th
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