Talk:Team:IvyTech-South Bend/2 September 2010

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9/2/10

Results from streaking

9/2/10

Protocol For Making LB-Broth/Agar Today I made 2 L LB/Agar I placed

1) 10 g LB-Lennox into 4 – 1000 mL capped Elenmyier Flasks

2) then placed/weighed 7.1 g Molecular Biology Agar into the same (4) Elenmyier Flasks

3) added 50 mL 18 ohm water into each of the 4 Elenmyier Flasks

4) then microwave on Beverage 8 oz. setting 6 times to melt /dissolve Agar into solution.

After dissolved I placed into Autoclave on Liquids Setting and let run overnight. note Irene made 250 mL and placed into Autoclave. I removed after done labeled 9/2/10 J.H. LB/Agar. and put in fridge in back.

9/2/10

I wasn’t able to fit all 4 – 1000 mL capped Elenmyier Flasks into Autoclave at one time so I placed the 2 in autoclave. and 2 in a 40 C Hot water bath.

The 2 in Autoclave we placed inside pan and set to Liquid setting and started which is in 121 C at 15 min. will autoclave the other 2

Friday 9/3/10 9/2/10 J.H.