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From 2010.igem.org

Contents 1 1：DNA Digestion 2 2:PCR 3 3：DNA extraction 4 4:Electrophoresis 5 5:Electrophoresis 6 6：DNA extraction from gels 7 7：DNA Digestion

1：DNA Digestion

＜Member＞
Hitomi

＜Sample, Materials＞
・PCR productions

• 1
• 2
• 4
• 5

＜Protocol＞
See Prptpcpl 9

2:PCR

<Member>
Hitomi

<Sample>(temperature in annealing)

• 7(67.5)
• 9(57,61,69)

<Protocol>
See Protocol 1

3：DNA extraction

＜Member＞
Hitomi

＜Sample＞
PCR products(9/23)

＜Protocol＞
See Protocol 4

4:Electrophoresis

<Member>
Hitomi

<Sample>
Digestion products.

• 1
• 2
• 3
• 4
• 5

<Protocol>
See Protocol 8

<Result>

5:Electrophoresis

<Member>
Hitomi

<Sample>
PCR products (9/23)

<Protocol>
See Protocol 8

6：DNA extraction from gels

＜Member＞
Mariko,Hitomi

＜Sample＞
Digestion products (9/23) (After electrophoresis)

＜Protocol＞
See Protocol 4

7：DNA Digestion

＜Member＞
Hitomi

＜Sample, Materials＞

• 2 (9/23)
• 4 (9/23)

＜Protocol＞
See Prptpcpl 9