"

From 2010.igem.org

2010/09/08

PCR

<Member>

Hitomi

<Sample>

• 5
• 1+2(ligation 2010/08/31)
• 4+5(ligation 2010/08/31)
• 7+8(ligation 2010/09/06)

<Protocol>

See Protocol2 (https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/)

• Tube(temperature in annealing)

1.(71.0,70.5,70.0℃)

2.(72.0,71.5℃)

3.(70.0℃)

4.(70.0℃)

Total 12 tubes.

Electrophoresis

<Member>

mizuki, Hitomi

<Sample>

PCR products.

• 5H
• 5M
• 5L
• 1-2H
• 1-2L
• 4-5
• 7-8

<Protocol>

See Protocol8

(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)

<Result>