From 2010.igem.org
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Cell Line
The cell line used in this project is the human embryonic kidney cell line 293T .
Databases Search
The [http://www.ncbi.nlm.nih.gov/sites/entrez?db=ncbisearch NCBI GEO] and [http://www.ebi.ac.uk/arrayexpress/browse.html ArrayExpress] databases were extensively searched for environmental conditions leading to apoptosis in 293T cells and genes differentially expressed under these conditions. The following data was obtained:
| Environmental Condition (EV)
| #genes selectively expressed in this EV
| Reference
|
| Conduritol B Epoxide treatment
| 57
| [1]
|
| Gammaherpesvirus
| 103
| [2]
|
| HIV integration
| 1
| [3]
|
| HLTV-1 p30
| 2291
| [4]
|
| Hypoxia
| 2
| [5]
|
| RCAS-beta-cateinS37A
| 55
| [6]
|
[1] Reference one Geo
[1] Reference one Geo
[1] Reference one Geo
[1] Reference one Geo
[1] Reference one Geo
[1] Reference one Geo
In order to understand gene regulation of apoptosis regulated genes we used the Transcriptional Regulatory Element Database [http://rulai.cshl.edu/cgi-bin/TRED/tred.cgi?process=searchPromForm (TRED)] to extract known promoters and the [http://string-db.org/ STRING] database for known interactions between genes and their transcription factors.
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