Team:UT-Tokyo/PCR（KODplus）

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UT-Tokyo

PCR(KODplus)

Components Volume Final Concentration 10× PCR Buffer for KOD -Plus- 5 μl 1× 2mM dNTPs 5 μl 0.2mM each 25mM MgSO4 2 μl 1.0mM Primer (10μM each) 1.5 μl 0.3μM each Template DNA ≧ 1 μl Genomic DNA 10~200 ng/50μl （Plasmid DNA 1~50 ng/50μl） KOD -Plus- (1U/μl) 1 μl 1 U/50μl MiliQ to 50 μl

Pre Denature : 94 degrees, 2min. Denature : 94 degrees, 15sec. Annealing : (Tm-5) degrees, 30sec. Extension : 68 degrees, 1min. /kb Set extension time for 1 min./kb

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