Talk:Team:UT-Tokyo

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Restriction Enzyme digestion —One by one


Team


Project -Sudoku --Abstract --construct --Lab note ---Parts Code ---Notebook:Main construct ---Notebook:Terminator leak switch ---Notebook:flpe ---Notebook:Virus expression ---Notebook:Anti-RNA repression --Result

-Allergy --Abstract --construct --Lab note

-Bioconcentration --Abstract --construct --Lab note


Parts -Check


Protocols -Transformation -Colony Pick Up -Miniprep -Restriction Enzyme Transaction(Xba1/Pst1)?Once for all -Restriction Enzyme Transaction ?One by one -PCR(Pfu Ultra) -PCR(Ex-Taq) -PCR(KODplus) -Colony PCR(Ex-Taq) -Electrophoresis -Gel DNA Recovery -LB broth -Agarose Gel -Sequencing -Producing Competent Cell -phosphorylation -phosphorylation & ligatibn

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