Protocols

Notice

-You should mix reagents well

-Put in enzymes after you mixed all other reagents

-Centrifuge the tube and collect substances on the bottom before opening a reagent

/Team:UT-Tokyo/Transformation

/Team:UT-Tokyo/Colony Pick Up

/Team:UT-Tokyo/Miniprep

/Team:UT-Tokyo/Restriction Enzyme digestion（Xba1/Pst1）—Once for all

/Team:UT-Tokyo/Restriction Enzyme digestion —One by one

/Team:UT-Tokyo/PCR（Pfu　Ultra）

/Team:UT-Tokyo/PCR（Ex-Taq）

/Team:UT-Tokyo/PCR（KODplus）

/Team:UT-Tokyo/Colony PCR（Ex-Taq）

/Team:UT-Tokyo/Electrophoresis

/Team:UT-Tokyo/Gel DNA Recovery

/Team:UT-Tokyo/LB　broth

/Team:UT-Tokyo/Agarose Gel

/Team:UT-Tokyo/Seaquencing

/Team:UT-Tokyo/Producing Competent Cell