Protocols

Notice

-You should mix reagents well

-Put in enzymes after you mixed all other reagents

-Centrifuge the tube and collect substances on the bottom before opening a reagent

Transformation Colony Pick Up

Miniprep

Restriction Enzyme Transaction（Xba1/Pst1）—Once for all

Restriction Enzyme Transaction —One by one

PCR（Pfu　Ultra）

PCR（Ex-Taq）

PCR（KODplus）

Colony PCR（Ex-Taq）

Electrophoresis

Gel DNA Recovery

LB　broth

Agarose Gel

Seaquencing

Producing Competent Cell