Team:Cambridge/Notebook/11

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Week 9: Monday 6th - Sunday 12th September

Monday

Theo made a video of the [http://www.youtube.com/watch?v=tUFscEVK5Ks bacterial bubble lamp], set up in the lab. He also attempted to ligate pBAD to EPIC luciferase, which had so far failed. He was pleased to find that he managed to change the colour of LC luciferase to green in a previous experiment, but had only a single colony so plated this out again.

Tuesday

To our surprise the EPIC pBAD was reddish, we made up a culture for sequencing to check that it was indeed EPIC, Theo performed a colony PCR which seemed to suggest it was. Ben and then Theo attempted to change the colour of LC luciferase from wildtype. Theo got good results for the attempt to change the 433rd amino acid, he performed Gibson assembly and transformation.

Theo

Wednesday

The ligation had failed so Emily and Hannah redid their experiment. Meanwhile, Ben attempted to use site-specific PCR mutagenesis followed by Gibson assembly to mutate the red mutant of Luciola cruciata luciferase back to wild type. This is a first step needed so that we can mutate it into a number of other colours.

Thursday

Friday