"

From 2010.igem.org

2010/09/08

PCR

<Member>

Hitomi

<Sample>

• 5
• 1+2(ligation 2010/08/31)
• 4+5(ligation 2010/08/31)
• 7+8(ligation 2010/09/06)

<Protocol>

See Protocol2 (https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/)

• Tube(temperature in annealing)

1.(71.0,70.5,70.0℃)

2.(72.0,71.5℃)

3.(70.0℃)

4.(70.0℃)

Total 12 tubes.

Electrophoresis

<Member> mizuki, Hitomi

<Sample>

<Protocol>

<Result>

File:Matsuura 2010-09-08 18hr 09min.tif