Protocols

Contents

• Preparation of Competent cells (E. coli DH5a)
• Bacterial Transformations
• Mini-prep (Alkaline SDS Method)
• PCR
• Restriction Enzyme Digestions
• DNA ligation
• Agarose gel electrophoresis
• Electroporation

Preparation of Competent cells (E. coli DH5a)

1. Day 1
   1. Single colony isolation on LB plate
   2. incubate the plate for 15-19 hrs at 37C
   3. lift a colony into 2 mL LB

Bacterial Transformations

Mini-prep (Alkaline SDS Method)

PCR

Restriction Enzyme Digestions

DNA ligation

Agarose gel electrophoresis