Team:HokkaidoU Japan/Protocols

From 2010.igem.org

Revision as of 15:01, 21 September 2010 by Sprkata (Talk | contribs)

Protocols

  • Preparation of Competent cells (E. coli DH5a)
  • Bacterial Transformations
  • Mini-prep (Alkaline SDS Method)
  • PCR
  • Restriction Enzyme Digestions
  • DNA ligation
  • Agarose gel electrophoresis
  • Electroporation

Preparation of Competent cells (E. coli DH5a)

Bacterial Transformations

Mini-prep (Alkaline SDS Method)

PCR

Restriction Enzyme Digestions

DNA ligation

Agarose gel electrophoresis

==Electroporation==