Team:HokkaidoU Japan/Protocols

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Protocols

Preparation of Competent cells (E. coli DH5a)
Bacterial Transformations
Mini-prep (Alkaline SDS Method)
PCR
Restriction Enzyme Digestions
DNA ligation
Agarose gel electrophoresis
Electroporation

Preparation of Competent cells (E. coli DH5a)

Bacterial Transformations

Mini-prep (Alkaline SDS Method)

PCR

Restriction Enzyme Digestions

DNA ligation

Agarose gel electrophoresis

==Electroporation==

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