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From 2010.igem.org

Visualization

The cells were placed in a 50 μm (?) high flow chamber (Details). The excitation for the bright field images (40x) was 60ms, the period something around 0.3s, aproximately corresponding to the fps rate of the movie. To increase speed, the binning in the microscope was set to 2, so that the images were 672 x 512 grayscale (16 bit) instead of the maximal resolution of the microscope of 1344 x 1024 pixels, and every pixel The images were made out-of-focus to simplify cell detection.